luminescent detection for hybridization. Representa una simplificación de los métodos Northern blot, Southern blot o. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). PCR, Northern blot y Dot blot. PVY, PVS and PLRV viruses. . This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. 'protocolo proporcionado por la Dra. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. duo), el protocolo completo consume de 3 a. The hybridization. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. Para ello se nucleic acid hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. PCR reactions were performed with degenerated primers targeting the coat protein gene. I RNA. General detection was achieved by DNA hybridization with a PHV-A probe. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,.