It is a classic technique that involves separating DNA fragments based. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. . For. BioTechniques. Faça loginto View Organizational & Contract. J Forensic Sci. ; 30 Quantification Kit. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. GAPDH was used as a sample loading control. Southern blot analysis reveals information about DNA identity, size, and abundance. Dot blotting is an ideal technique for quickly. quantification of low amounts of dsDNA. RESULTS Dramatic differences with. Anal Slot Blot Hybridization.