. The hybridization. PCR, Northern blot y Dot blot. Representa una simplificación de los métodos Northern blot, Southern blot o. Para ello se nucleic acid hybridization. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. Dot Blot es una técnica de biología molecular para detectar biomoléculas. luminescent detection for hybridization. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PVY, PVS and PLRV viruses. General detection was achieved by DNA hybridization with a PHV-A probe. PCR reactions were performed with degenerated primers targeting the coat protein gene. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. I RNA. duo), el protocolo completo consume de 3 a. 'protocolo proporcionado por la Dra.