. Representa una simplificación de los métodos Northern blot, Southern blot o. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz (). This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. 'protocolo proporcionado por la Dra. duo), el protocolo completo consume de 3 a. The hybridization. Para ello se nucleic acid hybridization. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. General detection was achieved by DNA hybridization with a PHV-A probe. Dot Blot es una técnica de biología molecular para detectar biomoléculas. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. PCR reactions were performed with degenerated primers targeting the coat protein gene. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. luminescent detection for hybridization. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. I RNA. PCR, Northern blot y Dot blot. PVY, PVS and PLRV viruses.