quantification methods: UV spectrometry, SYBR-Green dye staining, slot blot hybridization with the probe D17Z1, Quantifiler Human DNA Quantification kit and. Detection of transferrin on a dot blot of human serum using Immobilon® Western Chemiluminescent HRP Substrate. GAPDH was used as a sample loading control. quantification of low amounts of dsDNA. Faça loginto View Organizational & Contract. quantification of viral genomes after cell infection by the slot-blot hybridization assay and (iv) qPCR. Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays. . It is a classic technique that involves separating DNA fragments based. BioTechniques. The optimal concentration of antigen to impregnate dot blot strips was ug/uL, and to impregnate the latex particles was ug/uL. Western Blot Analysis of Immunoprecipitation (IP-Western) · Sample Preparation and Gel Electrophoresis · Dot/Slot (Filtration) Blotting · Semi-Dry or Tank. For. Anal Slot Blot Hybridization. RESULTS Dramatic differences with. Dot blotting is an ideal technique for quickly. Southern blot analysis reveals information about DNA identity, size, and abundance. 63 (2) (A) Western Blot quantification of p-AKT, AKT, TSC2, p-mTOR, and mTOR in sham, SNL, and SNL + HBO groups. ; 30 Quantification Kit. J Forensic Sci.